Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/3766
Title: GENOTYPING OF SALMONELLA STRAINS ISOLATED FROM DUCKS, THEIR REARING AND PROCESSING ENVIRONMENTS IN PENANG, MALAYSIA, USING RAPD
Authors: Adzitey, F.
Ali, G. R. R.
Huda, N.
Ahmad, R.
Keywords: Ducks
Genetic relatedness
Salmonella strains
RAPD
Issue Date: 2013
Publisher: Springer
Series/Report no.: Vol. 3;Issue 6
Abstract: Salmonella species are important foodborne pathogens that can cause illness and death in humans. The objective of this study was to determine the genetic relat edness of 115 Salmonella strains isolated from ducks and their environment using random amplified polymorphic deoxyribonucleic acid (RAPD). The analysis of Salmonella strains by RAPD produced DNA fingerprints of different sizes for differentiation purposes, and cluster analysis at a coefficient of 0.85 grouped the Salmonella strains into various clusters and singletons. S. Typhimurium were grouped into nine clusters and ten singletons, S. Hadar were grouped into seven clusters and nine singletons, S. Enteritidis were grouped into four clusters and five sin gletons, S. Braenderup were grouped into five clusters and four singletons, S. Albany were grouped into two clusters and seven singletons, and S. Derby were grouped into two clusters and four singletons at a coefficient of 0.85 with discriminatory index (D) ranging from 0.879 to 0.957. With the exception of S. Typhimurium strains which were grouped into three major groups (genotypes) by RAPD analysis, the rest were grouped into two major genotypes. RAPD was a useful genotyping tool for determining the genetic relatedness of the duck Salmonella strains. Com parison of the genetic relatedness among foodborne pathogens and their sources of isolation are important to trace their source and possibly the source of human infection.
URI: http://hdl.handle.net/123456789/3766
ISSN: 2190-572X
Appears in Collections:Faculty of Agriculture, Food and Consumer Sciences



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