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|Title:||A SIMPLE ASSAY OF PARACETAMOL BASED ON DRIED BLOOD SPOT SUITABLE FOR PAEDIATRIC PHARMACOKINETIC STUDIES|
|Authors:||Mohammed, B. S.|
Cameron, G. A.
|Series/Report no.:||Vol. 4;Issue 1|
|Abstract:||Dried blood spots in Guthrie cards are a reliable means of blood sampling suitable for pharmacoki-netic analysis in children. The aim of this study was to develop a simple and reliable bioanalytical method to measure the concentration of paracetamol in dried blood spots. Paracetamol was ex-tracted from dry blood spots by precipitation using 30% perchloric acid and separated on Hichrom 3.5 μ C18column. Detection was by a Waters 486 Tunable Absorbance Detector, at a wavelength of 244 nm. From five adult volunteers blood samples were collected as dried blood spots before and at 15, 30, 60, 120, 240 and 420 minutes after 1 g oral paracetamol was administered. Cmax, Tmax, t1/2 and CL/F were calculated using non-compartmental analysis. The standard curve for the method was linear in the range 0 – 100 μg ml-1. The LLOD and LLOQ were 550 pg and 1100 pg, respectively, on column. The median (min., max) Cmax, Tmax, t1/2 and CL/F, were 14.6 μg ml-1 (9, 19.1), 45 minutes (15, 60), 2.3 hours (1.1, 2.7) and 0.31 L kg-1 (0.21, 0.36), respectively. We report a simple assay for the analysis of paracetamol that can be used for monitoring blood concentration of paracetamol in young children.|
|ISSN:||2026 - 6294|
|Appears in Collections:||School of Medicine and Health Sciences|
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