Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/3626
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dc.contributor.authorWezena, C. A.-
dc.contributor.authorKrafczyk, J.-
dc.contributor.authorStaudacher, V.-
dc.contributor.authorDeponte, M.-
dc.date.accessioned2022-06-16T10:25:22Z-
dc.date.available2022-06-16T10:25:22Z-
dc.date.issued2017-
dc.identifier.issn0014-4894-
dc.identifier.urihttp://hdl.handle.net/123456789/3626-
dc.description.abstractThe redox metabolism of the malaria parasite Plasmodium falciparum and its human host has been suggested to play a central role for parasite survival and clearance. A common approach to test hypotheses in redox research is to challenge or rescue cells with pro- and antioxidants. However, quantitative data on the susceptibility of infected erythrocytes towards standard redox agents is surprisingly scarce. Here we determined the IC50 values of P. falciparum strains 3D7 and Dd2 for a set of redox agents using a SYBR green-based growth assay. Parasite killing in this assay required extremely high concentrations of hydrogen peroxide with a millimolar IC50 value, whereas IC50 values for tert-butyl hydroperoxide and diamide were between 67 and 121 mM. Thus, in contrast to tert-butyl hydroperoxide and the disulfide-inducing agent diamide, the host-parasite unit appears to be very robust against challenges with hydrogen peroxide with implications for host defense mechanisms. N-acetylcysteine, ascorbate, and dithiothreitol also had antiproliferative instead of growth-promoting effects with IC50 values around 12, 3 and 0.4 mM, respectively. So-called antioxidants can therefore also inhibit parasite growth with implications for clinical trials and studies on 'oxidative stress'. Furthermore, the addition of reductants to parasite cultures resulted in the gelation of albumin, the formation of methemoglobin and hemolysis. These effects can alter the fluorescence in SYBR green assays and have to be taken into account for the determination of IC50 values. In summary, standard oxidants and reductants both inhibit the growth of P. falciparum with IC50 values differing by three orders of magnitude.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesVol. 180;-
dc.subjectPlasmodiumen_US
dc.subjectErythrocyteen_US
dc.subjectMalariaen_US
dc.subjectIC50 valueen_US
dc.subjectRedoxen_US
dc.subjectAlbuminen_US
dc.subjectAscorbateen_US
dc.subjectDiamideen_US
dc.subjectDTTen_US
dc.subjectH2O2en_US
dc.subjectN-acetylcysteineen_US
dc.titleGROWTH INHIBITORY EFFECTS OF STANDARD PRO- AND ANTIOXIDANTS ON THE HUMAN MALARIA PARASITE PLASMODIUM FALCIPARUMen_US
dc.typeArticleen_US
Appears in Collections:Faculty of Biosciences



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