LACTOBACILLUS RHAMNOSUS INDUCED EPITHELIAL CELL APOPTOSIS, AMELIORATES INFLAMMATION AND PREVENTS COLON CANCER DEVELOPMENT IN AN ANIMAL MODEL

Abstract

Background/Aim: Probiotics have been suggested as prophylactic measure in colon carcinogenesis. This study aimed at determining the potential prophylactic activity of Lactobacillus rhamnosus GG CGMCC 1.2134 (LGG) strain on colorectal carcinogenesis via measuring its effect on Nuclear factor kappa B (NFkB) inflammatory pathway and apoptosis. Materials and methods: 64 Sprague Dawley rats were grouped into four as follows; Group 1 (Healthy control), Group 2 (LGG), Group 3 (cancer control Dimethyl hydrazine (DMH)) and Group 4 (LGG + DMH). LGG was administered orally to LGG and LGG + DMH groups. Colon carcinogenesis was chemically induced in LGG + DMH and DMH groups by weekly injection of 40 mg/kg DMH. Animals were sacrificed after 25 weeks of experiment and tumor characteristics assessed. The change in expression of NFkB-p65, COX-2, TNFa, Bcl-2, Bax, iNOS, VEGFa, b-catenin, Casp3 and p53 were evaluated by western blotting and qRT-PCR. Results: LGG treatment significantly reduced tumor incidence, multiplicity and volume in LGG + DMH treatment group compared to DMH cancer control group. Also, LGG treatment reduced the expression of b-catenin and the inflammatory proteins NFkB-p65, COX-2 and TNFa; the anti-apoptotic protein Bcl-2, but increased the expression of the pro-apoptotic proteins Bax, casp3 and p53 compared with DMH group. Conclusion: LGG have a potential protection effect against colon carcinogenesis; inducing apoptosis and ameliorating inflammation, and may hold a promise as bio-therapeutic dietary agent.