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http://hdl.handle.net/123456789/1067
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DC Field | Value | Language |
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dc.contributor.author | Helegbe, G. K | - |
dc.contributor.author | Huy, N.T. | - |
dc.contributor.author | Yanagi, T. | - |
dc.contributor.author | Shuaibu, M. N. | - |
dc.contributor.author | Kikuchi, M. | - |
dc.contributor.author | Cherif, M. S. | - |
dc.contributor.author | Hirayama, K. | - |
dc.date.accessioned | 2017-06-08T14:36:58Z | - |
dc.date.available | 2017-06-08T14:36:58Z | - |
dc.date.issued | 2013 | - |
dc.identifier.issn | 1475-2875 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/1067 | - |
dc.description.abstract | Background: Malaria anaemia is still a major public health problem and its pathogenesis still unclear. Interestingly, the progression of anaemia is at relatively low parasitaemia with some mortality in the semi-immune individuals in the endemic areas despite adequate erythropoietin (EPO) synthesis. A recent study has shown that treatment with exogenous anti-erythropoietin (anti-EPO) antibodies (Ab) of infected mice gives protection against malaria infection, suggesting an important role for anti-EPO Ab in malaria. The objective of the study was to evaluate anti-EPO antibody levels in anaemic condition of different strains of semi-immune mice with malaria. Methodology: Semi-immune status was attained in four mice strains (Balb/c, B6, CBA and NZW) by repeated infections with 104 Plasmodium berghei ANKA, and treatment with chloroquine/pyrimethamine. ELISA was used to measure anti-EPO Ab, transferrin and EPO while inflammatory cytokines measurement was done using bead-based multiplex assay kit. Results: The mean anti-EPO Ab levels in the mice strains [Optical Density (OD) values at 450 nm: Balb/c (2.1); B6 (1.3); CBA (1.4) and NZW (1.7)] differed (p = 0.045), and were significantly higher when compared with uninfected controls, p < 0.0001, and mean anti-EPO Ab levels in the mice strains at recovery [OD values at 450 nm: Balb/c (1.8); B6 (1.1); CBA (1.5) and NZW (1.0) also differed (p = 0.0004). Interestingly, EPO levels were significantly high in NZW and low in Balb/c mice (p < 0.05), with those of B6 and CBA of intermediary values. Again, NZW were highly parasitaemic (20.7%) and the other strains (Balb/c, B6 and CBA) ranged between 2.2-2.8% (p = 0.015). Anti-EPO Ab correlated positively with extent of Hb loss (r = 0.5861; p = 0.003). Correlation of anti-EPO antibody with EPO was significant only in Balb/c mice (r = −0.83; p = 0.01). Significant levels of IL6 and IFNγ (p < 0.0001), both known to be associated with erythropoiesis suppression were observed in the Balb/c. Transferrin was significantly lower in Balb/c (p < 0.0001) when compared with the other mice strains (B6, CBA and NZW). Conclusion: This is the first ever report in estimating endogenous anti-EPO antibodies in malaria anaemia. The data presented here suggest that anti-EPO Ab is produced at infection and is associated with Hb loss. Host factors appear to influence anti-EPO antibody levels in the different strains of mice. | en_US |
dc.language.iso | en | en_US |
dc.publisher | BioMed Central | en_US |
dc.relation.ispartofseries | Vol. 12;Issue.1 | - |
dc.subject | Anti-EPO antibody | en_US |
dc.subject | Erythropoietin | en_US |
dc.subject | Malaria anaemia | en_US |
dc.subject | Plasmodium berghei ANKA | en_US |
dc.subject | Semi immune | en_US |
dc.title | ANTI-ERYTHROPOIETIN ANTIBODY LEVELS AND ITS ASSOCIATION WITH ANAEMIA IN DIFFERENT STRAINS OF SEMI-IMMUNE MICE INFECTED WITH PLASMODIUM BERGHEI ANKA | en_US |
dc.type | Article | en_US |
Appears in Collections: | School of Allied Health Sciences |
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ANTI-ERYTHROPOIETIN ANTIBODY LEVELSAND ITS ASSOCIATION WITH ANAEMIA IN DIFFERENT STRAINS OF SEMI-IMMUNE MICE INFECTED WITH PLASMODIUM BERGHEI ANKA (2).pdf | 1.63 MB | Adobe PDF | View/Open |
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