CHARACTERIZATION OF SALMONELLA TYPHIMURIUM, SALMONELLA ENTERITIDIS AND SALMONELLA ALBANY ISOLATED FROM CHICKENS AND DUCKS USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)-PCRF

Abstract

The objective of this study was to characterize Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Albany strains isolated from chicken and ducks to determine their relatedness using Random Amplified Polymorphic Deoxyribonucleic Acid (RAPD)-PCR. RAPD-PCR analysis of the Salmonella serovars produced DNA bands that ranged from 242 to 3189bp for Salmonella Typhimurium, 252 to 2756bp for Salmonella Enteritidis and 232 to 2612 bp for Salmonella Albany. Cluster analysis at a coefficient of 0.85 grouped the Salmonella serovars into various clusters and singletons. Salmonella Typhimurium were grouped into 4 clusters and 1 singleton at a discriminatory index of 0.85.Salmonella Enteritidis were grouped into 2 clusters and 2 singletons at a discriminatory index of 0.64. Salmonella Albany were grouped into 3 clusters and 1 singleton at a discriminatory index of 0.71. One Salmonella Typhimurium isolated from chicken carcass was not characterized as the RAPD-PCR employed failed to produce any DNA band from that isolate. Characterizing Salmonella serovars from different sources is important to determine their genetic relatedness, and source of contamination and spread