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http://hdl.handle.net/123456789/3228
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DC Field | Value | Language |
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dc.contributor.author | Jing-Jing, W. | - |
dc.contributor.author | Walana, W. | - |
dc.contributor.author | Bing, W. | - |
dc.contributor.author | Jing, W. | - |
dc.contributor.author | Xia, L. | - |
dc.contributor.author | Jya-Wei, C. | - |
dc.contributor.author | John, G. R. | - |
dc.contributor.author | Jing-Min, L. | - |
dc.contributor.author | Fang, L. | - |
dc.date.accessioned | 2021-10-19T09:08:26Z | - |
dc.date.available | 2021-10-19T09:08:26Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 2227-4898 | - |
dc.identifier.uri | http://hdl.handle.net/123456789/3228 | - |
dc.description.abstract | ● AIM: To investigate the effect of interleukin-8 (IL-8) on neural retinal ganglion cells (RGCs) and whether it can be alleviated by G31P. ● METHODS: RGC-5 cells were exposed to IL-8 with or without its specific receptor antagonist G31P for 24h, and the cell viability was assessed by Cell Counting Kit 8 (CCK-8). Apoptosis was measured by examining nuclear morphology and quantifying with flow cytometry. Reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot were used to investigate the expression of apoptosis-related genes. ● RESULTS: CCK-8 assay showed that IL-8 significantly inhibits the viability of RGC-5 cells in a dose-dependent manner. Cell apoptosis assays exhibited higher apoptotic rate in IL-8 treatment group compared to control group. We further found that IL-8 could promote Bax and caspase-3 expressions, but decrease the level of Bcl-2 in the aspect of mRNA and protein. However, pre-treatment with G31P partly attenuated these effects in RGC-5 cells (P<0.05). ● CONCLUSION: These results indicate that anti-proliferation effects of IL-8 through induction of cell apoptosis regulated by Bcl-2, Bax and caspase-3 expressions, can be ameliorated by G31P. | en_US |
dc.language.iso | en | en_US |
dc.publisher | International journal of ophthalmology Press Co., Limited | en_US |
dc.subject | glaucoma | en_US |
dc.subject | inflammation | en_US |
dc.subject | interleukin-8 | en_US |
dc.subject | retinal ganglion cell-5 | en_US |
dc.subject | apoptosis | en_US |
dc.subject | G31P | en_US |
dc.title | CYTOTOXIC EFFECT OF INTERLEUKIN-8 IN RETINAL GANGLION CELLS AND ITS POSSIBLE MECHANISMS | en_US |
dc.type | Article | en_US |
Appears in Collections: | School of Medicine and Health Sciences |
Files in This Item:
File | Description | Size | Format | |
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CYTOTOXIC EFFECT OF INTERLEUKIN-8 IN RETINAL GANGLION CELLS AND ITS POSSIBLE MECHANISMS.pdf | 1.25 MB | Adobe PDF | View/Open |
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