Please use this identifier to cite or link to this item: http://hdl.handle.net/123456789/3138
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dc.contributor.authorWalana, W.-
dc.contributor.authorWang, J.-J.-
dc.contributor.authorYabasin, I. B.-
dc.contributor.authorNtim, M.-
dc.contributor.authorKampo, S.-
dc.contributor.authorAl-Azab, M.-
dc.contributor.authorElkhider, A.-
dc.contributor.authorKuugbee, E. D.-
dc.contributor.authorCheng, J. W.-
dc.contributor.authorGordon, J. R.-
dc.contributor.authorLi, F.-
dc.date.accessioned2021-06-24T12:12:50Z-
dc.date.available2021-06-24T12:12:50Z-
dc.date.issued2018-
dc.identifier.issn1879-1166-
dc.identifier.urihttp://hdl.handle.net/123456789/3138-
dc.description.abstractIL-8 is elevated during inflammation, and it initiates cascade of down-stream reactions. Its antagonist, CXCL8 (3–72) K11R/G31P (G31P), represses inflammatory reactions via competitive binding to CXC chemokine family, preferentially G protein-couple receptors (GPCRs) CXCR1/2. This study reports the effect of G31P on the transcription profile of lipopolysaccharide (LPS) induced inflammation in THP-1 monocytes ex-vivo. LPS (1 μg/ ml) induced elevation of IL-8 was significantly reduced by G31P (20 μg/ml and 30 μg/ml), with relatively increased inhibition of CXCR2 than CXCR1. Transcription of IL-1β, IL-6, and TNF-α were significantly inhibited, while IL-10 remained relatively unchanged. G31P treatment also had repressing effect on the inflammatory associated enzymes COX-2, MMP-2, and MMP-9. Significant restriction of c-Fos, and NF-kβ mRNA expression was observed, while that of c-Jun was marginally elevated. Conversely, SP-1 mRNA expression was seen to increase appreciably by G31P treatment. While the translation of pAKT, pERK1/2, and p65- NF-kβ were downregulated by the G31P following THP-1 cells stimulation with LPS, reactive oxygen species (ROS) expression was on the positive trajectory. Collectively, the IL-8 analogue, G31P, modulates the inflammatory profile of LPS induced inflammation in THP-1 monocytes via AKT1-NF-kβ and ERK1/2-AP-1 pathways.en_US
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.ispartofseriesVol. 79;Issue 11-
dc.subjectInflammationen_US
dc.subjectInterleukin 8en_US
dc.subjectCXCR1en_US
dc.subjectCXCR2en_US
dc.subjectTHP-1 monocytesen_US
dc.subjectLipopolysaccharideen_US
dc.titleIL-8 ANALOGUE CXCL8 (3-72) K11R/G31P, MODULATES LPS-INDUCED INFLAMMATION VIA AKT1-NF-kβ AND ERK1/2-AP-1 PATHWAYS IN THP-1 MONOCYTESen_US
dc.typeArticleen_US
Appears in Collections:School of Medicine and Health Sciences



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