CHARACTERIZATION OF S. TYPHIMURIUM, S. ENTERITIDIS AND S. ALBANY ISOLATED FROM CHICKENS AND DUCKS USING REPETITIVE EXTRAGENIC PALINDROMIC (REP) - PCR

Abstract

The objective of this study was to characterize Salmonella Typhimurium, Salmonella Enteritidis and Salmonella Albany strains isolated from chickens and ducks to determine their relatedness using Repetitive Extragenic Palindromic (REP)-PCR. REP-PCR analysis of the Salmonella serovars produced DNA bands that ranged from 253 to 7517 bp for Salmonella Typhimurium, 302 to 6746 bp for Salmonella Enteritidis and 241 to 7550bp for Salmonella Albany. Cluster analysis at a coefficient of 0.85 grouped the Salmonella serovars into various clusters and singletons. Salmonella Typhimurium were grouped into 3 clusters and 3 singletons at a discriminatory index of 0.85. Salmonella Enteritidis were grouped into 3 clusters and 1 singleton at a discriminatory index of 0.75. Salmonella Albany were grouped into 1 cluster and 8 singletons at a discriminatory index of 0.91. One each of Salmonella Typhimurium and Salmonella Enteritidis isolated from chicken carcass and mature egg, respectively was not characterized as the REP-PCR employed failed to produce any DNA band from that isolate. Characterizing Salmonella serovars from different sources is important to determine their relatedness, and source of contamination and spread.